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作 者 ：马建忠 梁厚果 邹喻萍 赵原

期 刊 ：植物学通报 1991年 8卷 03期 页码：45-50

关键词：绿豆子叶；衰老；呼吸作用；ADP／O；呼吸控制；ATPase；

摘 要 ：暗中培养的绿豆幼苗子叶在萌发后3—4天时，外观出现衰老征状，6天后子叶凋落。随子叶日龄的增加，子叶的呼吸强度一直下降，呼吸商始终小于1。当外加L—苹果酸、a—酮戊二酸、琥珀酸和NADH为底物测定离体线粒体氧化活性时，衰老子叶的线粒体对上述四种底物的氧化活性有不同程度的增加；抗氰呼吸也有所升高。子叶衰老时，线粒体的ADP／O和呼吸控制(RC值均降低)；线粒体ATPase水解ATP的活性升高。衰老绿豆子叶线粒体氧化磷酸化偶联效率的降低和ATPase水解活性的增强是与线粒体结构改变相联系的一种功能变化，它导致能量亏缺，并进一步加速了衰老的恶化进程。

Abstract:The respiratory metabolic changes in mung bean seedling cotyledons during senescence were as follows. (1) The respiratory rate of cotyledons of mung bean seedlings decreased rapidly during senescence in cotyledons because of a depletion of respiratory substrates in the cotyledons. (2) With the changes in mitochondrial structure of senescent cotyledons, the ADP/O and RC decreased. However, the oxidative activity of isolated mitochondria by adding L-malate, a-ketoglutarate, succinate and NADH and the cyanide-resistant respiration increased. (3) The catalitic activity of mitochondrial ATPase was transformed from synthesizing ATP into hydrolyzing ATP. The decay in oxidative phosphorylation and the transformation of ATPase were related with the changes of mitochondrial structure and resulted in the energy deficiency of the cotyledon cells and therefore accelerated the deteriorative processes of the cotyledon senescence.

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