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作 者 ：周思君 尹光初 雷勃钧 卢翠华 张开旺 钱华

期 刊 ：植物学通报 1992年 9卷 02期 页码：38-43

关键词：大豆；组织培养；体细胞胚胎发生；

摘 要 ：研究了影响大豆幼胚培养体细胞胚胎发生频率的9个因素。诱导体细胞胚胎发生的适宜幼胚长度为4mm；随着供体植株发育阶段的提高诱导频率下降；最适基本培养基为MS培养基; 蔗糖浓度从1.5％提高到9％，诱导频率逐渐下降；过高的维生素B1浓度对胚胎发生不利；2，4 — D的诱导效果优于NAA，适宜的2，4—D浓度为20ppm; 光、暗处理与生长素种类和浓度之间存在交互作用，接种方式对诱导频率影响很大，体细胞胚只在下表皮与培养基接触的幼子叶的上表皮上产生，当上表皮与培养基接触时，两个表皮都不能产生体细胞胚；被试的所有基因型都能被诱导胚胎发生，不同基因型的诱导频率存在差异。

Abstract:Nine factors which influence the frequency of somatic embryogenesis from immature embryos of soybean were studied. The best length of immatural embryo for somatic embryogenesis was 4 mm. Embryogenesis frequency decreased as the developing stage of the donor plant increased. The best medium was MS. The efficiency of embryogenesis decreased as the sucrose concentration increased from 1.5 to 9%. Overhigh concentration of vitamin B1 was not good for the somatic embrgogenesis. The initiation efficiency of 2,4-D was superior to that of NAA, the best concentration of 2,4-D was 20 ppm. There was on interaction between light-dark treatment and the type and concentration of auxin. To a great extent, the embryogenesis was influenced by ways in which the immatural cotyledons contacted the medium. Somatic embryos produced only on the adaxial surface of the cotyledons which contacted the medium. However, when the adaxial surface contacted the medium, both surfaces couldn‘t produce somatic embryos. Genotypic variation in the frequency of embryogenesis was noted. However, embryogenesis was induced from all tested genotypes.

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