全 文 ：SedativeandHypnoticEffectsofTartaryBuckwheat
EthanolExtract
HUYi-bing＊ , ZHAOGang, PENGLian-xin, ZOULiang
SchoolofBioindustry, ChengduUniversity, Chengdu610106
Abstract [ Objective] TheaimwastostudythesedativeandhypnoticefectsofTartaryBuckwheatethanolextractandlayafoundationforfur-
therexpandingtheapplicationofTartaryBuckwheat.[Method] TheefectsofTartaryBuckwheatethanolextractonpentobarbitalsodiumandmicespontaneousactiontimeswererecorded.[Result] EthanolextractofTartaryBuckwheatcouldprolongmicesleepdurationcausedbypen-
tobarbitalsodium(suprathresholddosage), increasemicesleepnumbercausedbypentobarbitalsodium(subthresholddosage), andobviously
inhibitmicespontaneousactiontimes.[ Conclusion] EthanolextractofTartaryBuckwheathasafunctionofsedation.
Keywords EthanolextractofTartaryBuckwheat;Sedation;Hypnosis;Mice
Received:November9, 2009 Accepted:December25, 2009
SupportedbyKeyProjectsintheNationalScience＆TechnologyPil-
lar Program during the Eleventh Five-year Plan Period
(2006BAD02B06)andProjectsinSichuanProvinceAdministration
ofTraditionalChineseMedicine(2006750).
＊Corespondingauthor.E-mail:huyibing123456@yahoo.com.cn
TheTartaryBuckwheatisdicotyledoninpolygonaceae
anditisuniqueresourcecropforedibleandmedicinaluse.
ThemedicinaluseofTartaryBuckwheathavebeenrecorded
inChineseMedicalBooks, inrecentyears, thegrain, root,
stem, leavesandflowerofTartaryBuckwheathavefla-
vonoids, whichhavemanyvariousactivitiessuchashypogly-
cemicefect, reducingbloodlipid, antioxidation, enhancing
immunity, scavengingfreeradicals, besides, theyalsohave
therapeuticeffectsondiabetesmelitus, hypertension, hyper-
lipidemiaandcoronaryheartdisease.However, thesedation
andhypnosisofethanolextractofTartaryBuckwheathavenot
beenreportedyet, whilethesedationandhypnosisofethanol
extractofTartaryBuckwheatarestudiedinthisexperimentto
layfoundationforexpandingclinicalapplicationofTartary
Buckwheat.
MaterialsandMethods
Materials
Drugs SomefulTartaryBuckwheatgrainswithhul(colect-
edfromLiangshanYiAutonomousPrefecture, SichuanProv-
ince)wereselectedforexperiment.Theyweregroundafter
dry, andthentheywererefluxedat85℃ by75% ethanoland
extractedtwicefor2 heverytime.Thefiltratesoftwicewere
combined, thentheethanolwasrecycledandthefiltrates
wereconcentratedtopasteshape.Thepastesweredriedat
80 ℃ toobtainextractforfutureuseandtheyieldratewas
6.67%.BunaoanshenSliceswereboughtfromWuhanJian-
minPharmaceuticalGroupCORP, LTD.Aldrugswerepre-
paredby0.5%CMC-Na.
Animals TheheathmaleKunmingmicewereweightedfrom
18 to22 gandthecertificationnumberwasSCXK(Chuan)
2008-14.
Reagents The pentobarbitalsodium wasboughtfrom
ShanghaiChemicalReagentFactory
Instrument ZZ-6 typemouselocomotoractivitywasbought
fromChengduTechnology＆MarketCo., LTD.
Methods
InfluencesofethanolextractofTartaryBuckwheaton
hypnotictimeofpentobarbitalsodiumtomice 40 male
miceweredividedintofourgroupsrandomlyandevenly,
namely, controlgroup, high-dosegroupofTartaryBuckwheat
ethanolextract, low-dosegroupofTartaryBuckwheatethanol
extractandBunaoanshenslicegroup.8 hbeforeexperiment,
themicewereonlywateredbutnotfed, duringexperiment,
themiceincontrolgroupwerefedisovolumetric0.5% CMC-
Na, whilemiceinlow-dosegroupofTartaryBuckwheatetha-
nolextractandhigh-dosegroupofTartaryBuckwheatethanol
extractwerefedwith0.13 and0.5 g/kgethanolextractof
TartaryBuckwheat, however, themiceinBunaoanshenslice
groupwerefedwith2.28 g/kgBunaoanshenslice.Almice
werefedwithdrugsforsuccessive7days;however, afterfed
drugsfor30min, themicewereinjected45 mg/kgpentobar-
bitalsodium(decidedbypreliminarytest).Thesleeptimeand
sleepdisappearancetimeweretaken, thenthesleeplatency
(sleepappearancetime-recordstartingtime)andsleeptime
andsleeptime(sleepdisappearancetime)werecalculated.
InfluencesofethanolextractofTartaryBuckwheaton
hypnoticdosageofmicecausedbysubthresholddosage
ofpentobarbitalsodium Thegroupingandmedicationad-
ministrationwereasthesameastheprevious.Afteradminis-
tratingdrugsfor30 min, micewereinjected35 mg/kgpento-
barbitalsodium(subthresholddosage), thenthesleepymice
wererecorded(afterinjectedfor15 min, themicewithout
rightingreflexwasrecordedassleep.)andthesleepingrate
ineverygroupwascalculated.
InfluencesofethanolextractofTartaryBuckwheaton
micelocomotoractivities[ 2] Thegroupingandmedication
administrationwereasthesameastheprevious.Afteradmin-
istratingdrugsfor30min, micewereputintolocomotoractivi-
tydeterminatortofor5 minadaptations, thenthelocomotor
activityin5 minwererecorded.
Datastatisticalmethod Thedatawereconductedinter-
groupttestandχ2 test.
ResultsandAnalysis
InfluencesofethanolextractofTartaryBuckwheaton
pentobarbitalsodiumhypnotictimetomice Compared
withcontrolgroup, theethanolextractofTartaryBuckwheat
MedicinalPlantAgriculturalScience＆Technology, 2010, 11(1):90-91Copyright 2010, InformationInstituteofHAAS.Alrightsreserved.
atlowandhigh-dosecouldprolongmicesleeptimewhich
werecausedbypentobarbitalsodium(Table1).
Table1 EfectsofTartaryBuckwheatethanolextractonhypnosis
timewithpentobarbitalsodium n=10
Group Doseg/kg Sleeplatencymin Sleepdurationmin
Blankcontrolgroup - 8.5±3.0 59.57±20.16
Low-dosegroupofTartaryBuckwheatethanolextract 0.13 7.8±2.6 79.70±20.89＊
High-dosegroupofTartaryBuckwheatethanolextract 0.50 6.1±1.5＊ 89.42±17.60＊＊
Bunaoanshenslicegroup 2.28 5.7±2.2 92.83±19.58＊＊
＊, ＊＊ standforsignificantdifferencesandextremelysignificantdiferencesat0.05, 0.01levelrespectivelycomparedwithblankcon-trol.Thesameasfolows.
InfluencesofethanolextractofTartaryBuckwheaton
hypnoticdosageofmicecausebysubthresholddosage
ofpentobarbitalsodium
Thesleepymiceandsleeprateinlow-dosegroupand
high-dosegroupwerehigherthantheseincontrolgroup.
Table2 EfectsofTartaryBuckwheatethanolextractonsubthresh-
oldhypnoticdosewithpentobarbitalsodium n=10
Group Doseg/kg Sleepinganimal Sleeprate∥%
Blankcontrolgroup - 1 10
Low-dosegroupofTartaryBuckwheatethanolextract 0.13 4 40
High-dosegroupofTartaryBuckwheatethanolextract 0.50 7 70＊
Bunaoanshenslicegroup 2.28 8 80＊＊
InfluencesofethanolextractofTartaryBuckwheaton
micelocomotoractivities
Comparedwithcontrolgroup, ethanolextractofTartary
Buckwheatathighconcentrationcoulddeclinenormalmice
activitynumbers(Table3).
Table1 EffectsofTartaryBuckwheatethanolextractonmicespon-
taneousactiontimes n=10
Group Doseg/kg Spontaneousactiontimes∥5times
Blankcontrolgroup - 25.5±7.6
Low-dosegroupofTartaryBuckwheatethanolextract 0.13 21.2±5.1
High-dosegroupofTartaryBuckwheatethanolextract 0.50 17.7±3.8＊＊
Bunaoanshenslicegroup 2.28 17.4±5.0＊＊
Discussions
TheTartaryBuckwheatisusedasfoodnormalyandit
hashighediblevalue.Theliteraturesinpastdynastiesalso
paymuchatentiontoediblerecord;however, themedicalre-
cordhasnotreferredinsomniatreatment.Itisfoundthatthe
TartaryBuckwheatmainlycontainsflavonoidsthroughmodern
drugresearchmeans, soitispredictedthatTartaryBuck-
wheathassedativeeffectthroughcombiningpreviousstudies
ofanalgesictest.Theexperimenthasdemonstratedthatthe
ethanolextractofTartaryBuckwheatcanprolongsleeptimeof
micebelowthresholdconcentrationandincreasethesleepy
mice;besides, italsocandeclinelocomotoractivitiesof
mice, sotheTartaryBuckwheathassedativeandhypnotic
efect.
References
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Responsibleeditor:CHENXiu-chen Responsibletranslator:LIZhu-le Responsibleproofreader:WUXiao-yan
苦荞醇提物的镇静催眠作用研究(摘要)
胡一冰＊ ,赵 钢 ,彭镰心 ,邹 亮 (成都大学生物产业学院 ,四川成都 610106)
[目的]研究苦荞醇提物的镇静催眠作用 ,为进一步扩大苦荞临床应用奠定基础。[方法]将颗粒饱满的苦荞带壳种子干燥后适度研碎 , 75%乙醇(固液比 1∶10), 85℃回流提取 ,提取 2次 ,每次 2h,合并 2次滤液 ,回收乙醇并浓缩至稠膏状 ,经 80℃烘干 ,得苦荞提取物 ,提取物得率为 6.67%。记录苦荞醇提物对戊巴比妥钠引起的小鼠睡眠持续时间 、入睡率及小鼠
自主活动次数。将 40只雄性小鼠随机分为 4组 ,每组 10只 ,分别为空白对照组、苦荞醇提物高剂量组 、苦荞醇提物低剂量组和阳性药物对照组;试验前 8h禁食不禁水 ,试验时空白对照组给予等体积的 0.5% CMC-Na,苦荞低剂量组、高剂量组分别给 0.13g/kg、0.5g/kg苦荞醇提物 ,阳性药物对照组给 2.28g/kg的补脑安神片;连续给药 7d。①末次给药 30min后 ,小鼠腹腔注射戊巴比妥钠 45mg/kg(由预试验确定),记录睡眠出现和消失的时间 ,计算睡眠潜伏期(睡眠出现时间 -记录开始时间),睡眠持续时间(睡眠消失时间-睡眠出现时间);②末次给药
后 30min,小鼠腹腔注射戊巴比妥钠 35mg/kg(戊巴比妥钠阈下催眠量 ,即 90%～ 100%小鼠不入睡的戊巴比妥钠最大剂量 ,由预试验确定),观察并记录入睡动物数(注射后 15min内小鼠翻正反射消失达 1min以上者记为入睡),计算各组入睡率;③末次给药 30min后将小鼠放入小鼠自主活动测定仪中 ,让其适应 5min,然后开始测定 5min内自主活动次数。[结果]苦荞醇提物能延长戊巴比妥钠阈上剂量引起的小鼠睡眠持续时间 ,增加戊巴比妥钠阈下剂量引起的小鼠睡眠只数 ,且能明显减少小
鼠自主活动次数。[结论]苦荞醇提物具有镇静作用。关键词 苦荞醇提物;镇静;催眠;小鼠
基金项目 国家十一五科技支撑计划重点项目(2006BAD02B06);四川省中医药管理局资助项目(2006750)。
作者简介 胡一冰(1974-),女 ,贵州凯里人 ,博士 ,讲师,从事疾病动物模型与中药复方药理研究。 ＊通讯作者。
收稿日期 2009-11-09 修回日期 2009-12-25
91HUYi-bingetal.SedativeandHypnoticEfectsofTartaryBuckwheatEthanolExtract