Abstract:In this paper, arbuscular mycorrhizal fungi (AMF) Glomus versiforme (GV), Glomus mosseae(GM),Glomus intraradices(GI) and their mixtures were applied to inoculate plastic greenhouse Cucumis sativus seedlings to investigate the effects of AMF on C. sativus growth, development, yield, and quality. The results showed that all test AMF could form mycorrhiza with cucumber roots, and the infected rate reached 41.74%~55.69%. Compared with the control, treatments GV, GM, GM+GV, GM+GV+GI and GV+GI enhanced the healthy seedling index by 58.14%~123.6%,and increased early yield by 21.71%~ 37.87% and total yield by 19.72%~34.41%. AMF also improved fruit quality. The Vc content of cucumber fruit increased by 22.84% and 21.95% in treatments GM+GV and GV+GI, soluble sugar content increased by 13.29%, 8.25%, and 10.20% in treatments GV, GI and GV+GI, and amino acid content increased by 47.66% and 23.19% in treatments GV and GM+GI, respectively, while soluble protein content increased by 17.67%~34.29% in all AMF treatments. The results suggested that AMF inoculation could significantly promote the seedling growth of cucumber and improve its fruit quality, and the effects differed with different AMF and their combinations.