全 文 ：第27卷第5期
2008年10月
生态科学
EcologicalS ience
27(5)：424-426
OCt．2008
EffectsofExtractsfromGracilarialem neifoFmon
Mieroalgae
LUHui．min91’2，LIAOXiao-jian3，YANGYu．fen91，2’，XUShi．hai3
1InstituteofHydrobiology,JinanUnivers ty,
2KeyLaboratoryoftheEutrophicationofWaterBodyandRedTideControl,DepartmentofEducationof
GuangdongProvince
3Departmentofchemistry,dinanUniversity,Guangzhou510632，China
Keywords．Gracilarialemane form；Skeletonemacosta um；allelopathy；semi—effectiveconcentration； utrophication
大型海藻龙须菜浸出组分对中肋骨条藻的化感抑制作用
卢慧明地，廖小建3，杨宇峰1，扩，徐石海3
1暨南大学水生生物研究中心，
2水体富营养化与赤潮控制广东省教育厅重点实验室，
3暨南大学化学系， 广州510632
【摘要】 研究了龙须菜乙醇浸出组分对中肋骨条藻的化感抑制作用。结果显示，浸出组分对中肋骨条藻产生明显
抑制作用，且微藻的生物量迅速减少。当龙须菜浸出成分浓度为42mgLl时，相对抑制率为50％，即半效应浓度(EC∞)。
龙须菜对赤潮藻产生明显的化感抑制作用，延缓了富营养化水体中赤潮藻的爆发，从而使龙须菜具有控制中肋骨条
藻赤潮发生的潜在价值。
关键词：龙须菜；中肋骨条藻；化感作用；半效应浓度；富营养化
中图分类号：Q仃8．53文献标识码：A文章编号：1008．8873(2008)05-424—03
1Introduction
Plantsinteractmongthemselvesphysically，but
moreoftenbychemicalmeans．Onesuchchemical
interactionis allelopathyandonesuchchemical
substanceiS allelochemical．IntheFirstWorld
CongressonAllelopathy【IJ．allelopathywasd fined勰
‘Anyprocessthatinvolvess condarymetabolites
producedbyplants，algae，bacteriaandfungth t
influencethegrowthandevelopmentofbiological
sys ems’．Someaquaticmacrophytesshowallelopathy
toothermicroalgaes，andcanreducebiomassof
microalgaesbyreleasingallelopathiccompoundst2]．
Thispresentworksis to studyeffectsofG．
1emaneiformandassessthepotentialoftheseaweedG．
1 maneiform．
收稿日期：2008—09-10收稿，2008一10一10接受
基金项目：国家基金一广东省联合重点基金(U0633006)。863项目(2007AA092436)，广东省高校重点项目(2007129)
作者简介：卢慧明(1981一)，男，博士研究生，河北石家庄人，研究方向：海洋环境与赤潮。
‘通讯作者，E．mail：tyyf@jnu．edu．ca
万方数据
5期 卢慧明，等：大型海藻龙须菜浸出组分对中肋骨条藻的化感抑制作用 425
2MaterialsandMethods
2．1Collectionofsamples
SamplesofG．1emaneiforrawerecollectedfromthe
NanAoIsland，GuangdongProvince．
2．2Algaculture
111eaxenicstrainofG．1emaneiformwasobtained
fromtheAlgaeCultureCollection，Instituteof
Hydrobiology，JinanUniversity．Priortotheinitiation
oftheexperiments．short—termbatchulturesofG．
1emaneiformweregrownin100mlconicalflasksfilled
with60mlf／2medium．Lightsourcewasuppliedby
fluorescenttubesofonesidewithadaylightsimilar
emission．Spectrum(60I_tmolm-2s-1)at12：12L／Dcycle．
Thetemperaturewasmaintainedat20士l℃ina
air-conditionedgr wthchamber．Cellsin the
exponentialgrowthphasew recollectedfrombatch
culturesandusedastheinoculaforthefollowing
experiments．
2．3PreparationextractsofGlemaneiform
G．1emaneiform(2．3kgdryweight)wascutinto
unifo册lengths(2cm)．Thesefragmentswere
immersionedna hydrousalcohol(40kg)for30d．The
extractswereconcentratedto50mlat50℃under
reducedpressureinanevaporator．Theconcentrated
extract(50m1)wasfilteredthroughaW atmanGF／C
filter．Theseextractswerethenstoredat．20。Cuntil
required．
2．4Dataprocessing
Therelativenhibitoryrateofextractsfrom
G．1emaneiformonalgaegrowth【jJ-
IR(％)=(1-T／C)x100％ (1)
IR--relativeinhibitoryrate，T--treatmentgroup，C
--controlg up
howevertreatmentalgaegrowthsuffereda certain
influencea dgrowthcurvealsohaschanged．Andthe
concentrationofalgaeisatthelowlevela lthetime．
Theresultshowsthatallelochemicalcouldsignificantly
inhibitalgaegrowthQ<0．01)．42mgL“ofextracts
fromG．1emaneiformsh wsthatherelativenhibitory
rateis50％(ECso)141
3．2EffectofextractsfromGlemaneiformonalgae
cellmorphology
Themicro．measurementindicatesthat hecell
volumeofthetreatmentalgaeisbiggerthanthatofthe
control，
unclearbo dersandthecolorfthecellsi hardly
removed．Fromwhichwecaninferthatcellstructureis
lysedgradually．
3．3discussion
Thegrowthof&costatumatcorrespondinginitial
celldensityissignificantlyreducedinthepresence
extractsofG．1emaneiform．Ingeneral，mostcoexistence
studieshavedemonstratedallelopathieeffectofmany
macrophytesonalgae【5捌，butthemechanismispoorly
understood．StudiesusingEscherichiacoliliposomes
haveshownthattheffectoferpenesonthestructural
andfunctionalpropertiesofbiologicalmembraneswas
directlyre atedoaccumulationinthesem mbranes，the
effectwasindependentofthes ructuralfe turesofthe
molecules[71．Effectsincludedswellingofthe
membranebilayer,changedinmembranefluidityand
increasedpassivefluxofprotons．Otherstudieshave
shownthaterpeneshavetheabilitytodisruptor
penetratelipidstructures【81．
Inconclusion，allelopathyisaquitecomplexsystem
engineeringproblem,whichrelatestomanyindex
factorsf ignalstransductionpathway,actionsite，
ablationofallelochemicalsubstances，changesincell
structureandorganelle．
3 ResultsandDiscussion
References
3．1EffectofextractsfromGlemaneiformonalgae
growth IllAnayaAL．1999．Allelopathyasatoolinthemanagement
Modelofogisticsuitableforcontrolalgaegrowth, ofbioticresourcesinagroecosysterns[J]．CriticalReviews
万方数据
426 生态科学EcologicalScience 27卷
12l
【3l
15l
inPlantSciences．18：697—739．·
ErhardD，GrossEM．2006．AllelopathieacuityofElodca
canadensisandElodeanuttalliiagainstepiphytcsand
phytoplankton[J]．AquaticBotany，85：203-211．
WilliamsonGB，RichardsondD．1988．Bioassayfor
Allelopathy：MeasuringTreatmentresponseswith
IndependentControls[J]．JournalofChemicalEcology,
14(1)：181-187．
HayesAW．1989．Principles&MethodsofToxicology．
2埘Ed【M】．．NewYork：RavenPress．
LurlingM，GeestGV，SchefferM．2006．Importancef
nutrientcompetitionandallelopathiceffectsinsuppression
ofthegreenalgaScencdesmusobliquusby the
macrophytcsChara，ElodeaandMyriophyllum[J]．
Hydrobiologia556：20争-220．
MulderijG．2006jChemicalwarfareinfreshwater．PhD
Thesis[D]．RadboudUnivcrsiteitNijmegen：157—170．
SikkemaJ，BontD，PoolmanJAB．1994．Interactionsof
cyclichydrocarbonswithbiologicalmembranes[J]．Journal
ofbiologicalchem妇try，269：8022—8028．
TakahashiILHosakaM，HanabusaY，et／．1996．
Dispersionofstratumcorneunlinaqueousmixedsolutions
ofsurfactantandterpene：theeffectofmixturesofN’j呵一
dimethyldo-decylamineoxideand【alpha]-Terpineol[J]．
Co／／o／dsandsurfaces,109：273-282．
万方数据
大型海藻龙须菜浸出组分对中肋骨条藻的化感抑制作用
作者： 卢慧明， 廖小建， 杨宇峰， 徐石海， LU Hui-ming， LIAO Xiao-jian， YANG Yu-feng
， XU Shi-hai
作者单位： 卢慧明,杨宇峰,LU Hui-ming,YANG Yu-feng(暨南大学,水生生物研究中心;水体富营养化与
赤潮控制广东省教育厅重点实验室)， 廖小建,徐石海,LIAO Xiao-jian,XU Shi-hai(暨南大
学,化学系,广州,510632)
刊名： 生态科学
英文刊名： ECOLOGICAL SCIENCE
年，卷(期)： 2008,27(5)
被引用次数： 3次

参考文献(8条)
1.Takahashi K;Hosaka M;Hanabusa Y Dispersion of stratum corneum in aqueous mixed solutions of
surfactant and terpene:the effect of mixtures of N,N-dimethyldo-decylamine oxide and[alpha]-
Terpineol[外文期刊] 1996
2.Sikkema J;Bont D;Poolman J A B Interactions of cyclic hydrocarbons with biological membranes 1994
3.Mulderij G Chemical warfare in freshwater 2006
4.Lurling M;Geest G V;Scheffer M Importance of nutrient competition and allelopathic effects in
suppression of the green alga Scenedesmus obliquus by the macrophytes Chara,Elodea and Myriophyllum
[外文期刊] 2006(1)
5.Hayes A W Principles & Methods of Toxicology 1989
6.Williamson G B;Richardsond D Bioassay for Allelopathy:Measuring Treatment responses with
Independent Controls[外文期刊] 1988(01)
7.Erhard D;Gross E M Allelopathic activity of Elodea canadensis and Elodea nuttallii against
epiphytes and phytoplankton[外文期刊] 2006
8.Anaya A L Allelopathy as a tool in the management of biotic resources in agroecosystems[外文期刊]
1999(6)

引证文献(3条)
1.边归国.赵卫东.达来 农副产品及废弃物化感抑藻作用的研究与机理[期刊论文]-内蒙古农业大学学报（自然科学
版） 2012(2)
2.别聪聪.李锋民.李媛媛.赵雅菡.王震宇 六种大型藻浸提液对中肋骨条藻的抑制及活性成分分离[期刊论文]-中国
海洋大学学报（自然科学版） 2011(7)
3.邵旻玮.孙雪.徐年军 龙须菜对赤潮藻的生长抑制效应及其与环境因子的关系[期刊论文]-海洋学研究 2011(2)


本文链接：http://d.g.wanfangdata.com.cn/Periodical_stkx200805032.aspx