Abstract:During strawberry (Fragaria ananassa Duch cv.chunxing)fruits maturation,the content of total sugar,anthocyanin and respiration rate increased significantly,and Ca2+-ATPase activity of cytoplasm and microsome membrane increased firstly,then peaked at pink stage,and decreased at full red stage in fruits,and also,the activity of plasma membrane Ca2+-ATPase was the highest in microsome membranes. Na3VO4,an inhibitor of plasma membrane Ca2+-ATPase activity,stimulated accumulation of anthocyanin,declined the total sugar content,but had different effects with various fruit mature degree.