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作 者 ：郭建军 叶庆生 李玲

期 刊 ：植物学通报 2002年 19卷 01期 页码：63-69

关键词：GAs；禾谷类；α-淀粉酶；基因表达；信号转导；

摘 要 ：用GAs处理禾谷类糊粉细胞原生质体后，可以诱导α_淀粉酶的合成与分泌。ABA抑制GAs的诱导作用并可刺激ABA诱导蛋白的产生。GAs和ABA的受体位于质膜上。最近的研究表明：G蛋白、cGMP、Ca2+和钙调素、三磷酸肌醇（IP3）及蛋白质磷酸酶（PP1和PP2A）都不同程度的参与了GA响应的信号传导过程。已克隆出一些可在转录水平上调节GA诱导基因的顺反子，并证明它们在禾谷类糊粉细胞中的GA响应事件中起至关重要的作用。有证据表明GA在α淀粉酶的转录后水平的调节上也有作用。

Abstract:The synthesis and secretion of α_amylases was induced by gibberellins (GAs) in the protoplasts of cereal aleurone layer cells.ABA repressed the effects of GA and stimulated the production of ABA_up_regulated proteins. Receptors for GA and ABA had been localized on the aleurone cell PM. Recent researches proved that G-p rotein,cGMP,Ca2+ and CaM,IP3 and protein phosphatases 1 and 2A were in volved in the signal transduction pathways of GA response event. Some cis_acting elements and trans_action factors which regulate GA_induced genes at the transcription level were cloned. They played an important role in the GA response event of the cereal aleurone cells. Some evidences showed that GA had effects on the post-transcription level of α-amylases.

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