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作 者 ：彭建宗 陈兆平 程双奇

期 刊 ：植物学通报 2000年 17卷 03期 页码：266-269

关键词：凝集素；洋紫荆；亲和层析；纯化；

摘 要 ：利用酸化处理的Sepharose 6B 亲和柱从洋紫荆种子中分离纯化出了洋紫荆凝集素(BVL)，其比活性比抽提液提高了159倍，活力回收率为49.0%。BVL分子量为81 000，由两个相同的亚基组成。等电聚焦凝胶电泳测得其等电点为4.95。其紫外吸收高峰在276 nm处。BVL具一定的热稳定性和酸碱稳定性。N-乙酰-D-氨基半乳糖能强烈地抑制BVL对兔红细胞的凝集作用，乳糖、半乳糖也有较强的抑制作用。

Abstract:Bauhinia variegata lectin（BVL） has been purified from the seed of Bauhinia variegata L. by affinity chromatography on an acidtreated Sepharose 6B column . The hemagglutinating activity of the purified BVL increased 159 times and the activity recovery is 49.0%. The molecular weight is 81 000 and the molecule consists of two identical subunits. Its isoelectric point is 4.95. NacetylDgalactosamine is the most potent inhibitors of BVL. Lactose and galactose can also inhibit the agglutination of rabbit erythocytes by BVL.

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