---

作 者 ：李丽 张景昱 杜桂森 宋艳茹

期 刊 ：植物学通报 2001年 18卷 02期 页码：216-220

关键词：种子特异性启动子；植物表达载体；β-葡萄糖酸苷酶；转基因；

摘 要 ：利用PCR技术从油菜Brassica napus H165基因组DNA中分离了napinB启动子。序列分析表明，扩增片段(nap300)与文献报道的napinB启动子相应区域的同源性为97%。将其与gus连接构建种子特异性表达载体，农杆菌介导转化烟草。PCR、Southern结果显示，nap300已整合到烟草基因组DNA中，获得了转基因植株。

Abstract:A fragment of 300bp (nap300) in napinB promter region was isolated by PCR from genomic DNA of Brassica napus H165. Nap300 and the corresponding sequence of napinB promoter shared 97% homology. Expression vector harboring the promoter fused with GUS gene was constructed for functional analysis. Tobacco plants were transformed by Agrobacterium tumefaciens-mediated method. PCR and Southern results showed that nap300 had been integrated into genomic DNA of tobacco successfully. Assay of GUS activity in the seeds of transgenic tobacco will be carried out to identify the function of the promoter.

---