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作 者 ：梁机 陈晓阳 林善枝 谢响明

期 刊 ：植物学通报 2002年 19卷 06期 页码：650-658

关键词：发根农杆菌；Ri质粒；rol基因；表型变异；林木遗传改良；

摘 要 ：Ri质粒上携带的诱根基因的表达不仅能导致植物被感染部位形成大量的毛根，而且由毛根容易获得再生的转化植株，这些植株可表现出许多能稳定遗传的表型变异，在植物遗传改良中有着广阔的应用前景。20世纪80年代以来，国内外学者对发根农杆菌Ri质粒及其rol基因进行了广泛深入的研究。本文重点综述了农杆碱型发根农杆菌Ri质粒的结构与功能，rol基因的位点与特征，Ri质粒的转化与rol基因的表达对植物生长发育的影响及在林木遗传改良上应用等方面的研究现状，并讨论了Ri质粒rol基因在林木遗传改良应用上存在的问题与应用前景。

Abstract:Expression of rol genes from Ri plasmid leads to the excessive formati on of adventitious roots at the site of infection in plant, and transformed plants can be easily regenerated from the adventitious roots. The plants transformed with Ri plasmid rol genes often generate various genetically modified characteristics which have broad prospects for the application of plant genetic improvemen t. Since the 80‘s of the last century, much progress has been made in the studies on agropine type Ri plasmid rol genes and their applications for plant genetic improvement, which involves the structure and function of Ri plasmid, the characters of rol genes, the influence of rol genes expression on plants growth and development, and the applications of rol genes for forest tree genetic improvement. In this paper, the advances in this field are reviewed and the existing problems about the application ofrolgenes on forest tree genetic improvement are also discussed.

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