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作 者 ：崔红 丁群星 桂耀林 郭仲琛

期 刊 ：植物学通报 1999年 16卷 04期 页码：411-415

关键词：甘薯；体胚发生；悬浮培养；过氧化物同工酶；

摘 要 ：将来源于‘徐薯18’叶片的胚性愈伤组织，接种在含有不同2,4-D浓度的液体MS培养基中进行悬浮培养，悬浮细胞表现出不同的形态结构、分裂方式和发育途径：2,4-D浓度为1 mg/L时，细胞均等分裂，增殖迅速；不含2,4-D时，细胞多进行不均等分裂，并发育成体细胞胚。不同2,4-D浓度中培养的悬浮细胞，其胞外过氧化物同工酶谱及其随时间变化的方式有很大差异，并与细胞的生长、发育过程密切相关。

Abstract:Divergences were discovered in cell morphology, mode of cell division and passage of cell differentiation, when embryogenic calli induced from leaves of ‘Xushu 18’ sweet potato were suspension-cultured in modified MS medium with different concentrations of 2, 4-D. Cells grew quickly and divided equally in medium containing 1 mg/L 2,4-D. When 2,4-D was deleted, the cells divided unequally and developed into embryos. Extracellular pemxidase isozyme patterns changed greatly during suspension culture with different concentrations of 2,4-D and were closely related to cell development process.

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