The synthetic rate of proteins was lower at high temperature stress （42 ℃）than that at normal condition（25 ℃）. Seeds with high vigor synthesized more heat shock proteins (HSPs) during germination. Five kinds of HSPs were found at 42 ℃ in maize (Zea mays L.) seeds, and their molecular weight was 73 kD, 65 kD, 62 kD, 54 kD and 18 kD respectively. Quantitative difference was found between seeds with high and low vigor. Seeds with high vigor had a special HSP of 56 kD, this might be used as an index of seed vigor. 2D-PAGE showed more qualitative difference between seeds with high and low vigor for the synthesis of HSPs.
光照和温度对豌豆Lhcb2 基因表达的影响
孙钦秒 李良璧* 阎久胜 毛大璋 匡廷云
（中国科学院植物研究所光合作用研究中心，北京100093）
摘要：采用RT-PCR技术 ,从豌豆 (Pisumsativum L .)幼叶中克隆了 1个约 80 0bp的Lhcb2cDNA。以特异探针进行的Southern杂交结果表明 ,Lhcb2基因以单拷贝形式存在于豌豆基因组中。不同光照时间和温度对豌豆幼苗进行处理的RT_PCR和Northernblotting分析表明 ,Lhcb2基因转录水平上的表达受光照的控制 ,且明显地表现出对光照时间的依赖性。光照 0～ 1.5hLhcb2基因未见表达 ,而光照 2h以上该基因才大量表达 ;温度对Lhcb2基因的表达亦有显著的影响 ,相同的光照处理 ,4℃下Lhcb2基因的表达量比 2 5℃下的表达量低 1倍左右。
关键词： 豌豆；Lhcb2基因；光照和温度；转录表达
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