By mild PAGE method, 11, 11, 7 and 9 chlorophyll-protein complexes were isolated from two species of siphonous green algae (Codium fragile (Sur.) Hariot and Bryopsis corticulans Setch.), green alga (Ulothrix flacca (Dillw.) Thur.), and spinach (Spinacia oleracea Mill.), respectively. Apparent molecular weights, Chl a/b ratios, distribution of chlorophyll, absorption spectra, low temperature fluorescence spectra of these complexes were determined, and compared with one another. PSⅠ complexes of two siphonous green algae are larger in apparent molecular weight because of the attachment of relative highly aggregated LHCⅠ. Four isolated light-harvesting complexes of PSⅡ are all siphonaxanthin-Chl a/b-protein complexes, and they are not monomers and oligomers like those in higher plants. Especially, the absence of 730 nm fluorescence in PSⅠ complexes indicates a distinct structure and energy transfer pattern.
管藻目绿藻叶绿素蛋白复合物特性及比较研究
陈敏1* 李爱芬1 周百成2
（1. 烟台大学生物化学系，烟台264005；2. 中国科学院海洋研究所，青岛266071)
摘要：采用温和的PAGE法从管藻目刺松藻 (Codiumfragile (Sur.)Hariot)和假根羽藻 (Bryopsiscorticulans Setch .) ,丝藻目绿藻软丝藻 (Ulothrixflacca (Dillw .)Thur.) ,及菠菜 (SpinaciaoleraceaMill.)中分别得到 11、11、7和 9种色素蛋白复合物 ,对复合物的多种特性 ,包括分子量、Chla/b比值、叶绿素的相对分布、吸收光谱、77K低温荧光光谱等进行了系统的测定和比较。管藻目绿藻的PSⅠ含有聚集程度更大的LHCⅠ ,因而分子量较大 ;分离出的 4种主要的PSⅡ捕光复合物都是管藻黄素_Chla/b_蛋白复合物 ,并且相互之间不像高等植物那样为单体和寡聚体的关系 ;特别是其PSⅠ复合物的低温光谱中缺少 730nm特征峰。表明这类藻的光系统在结构及能量传递方式方面都具有特殊性 .
关键词： 叶绿素蛋白复合物；PACE；管藻目；绿藻
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