RFLP (restriction fragment length polymorphism) was employed to analyze cytoplasmic genome of diploid somatic hybrid plant, morphologically similar to rough lemon which was leaf parent, that was produced via protoplast fusion between rough lemon (Citrus jambhiri Lush) and Hamlin sweet orange (C. sinensis Osb.), the embryogenic parent. Three enzyme-mitocondrial probe combinations and one enzyme-chloroplast probe combination demonstrated that the plant had identical band profiles to Hamlin sweet orange as far as mtDNA and cpDNA were concerned.
粗柠檬与哈姆林甜橙二倍体体细胞杂种胞质基因组初步分析
刘继红 邓秀新*
（华中农业大学作物遗传改良国家重点实验室，武汉430070）
关键词： 柑橘；胞质杂种；RFLP；线粒体基因组
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