Achyranthes bidentata polysaccharides (ABPS), water soluble polysaccharides, isolated from the roots of Achyranthes bidentata Bl. of Amaranthaceae family, was divided into four parts, named as Con.1, Con.2, Con.3 and Con.4, respectively, by chromatography on DEAE Sepharose fast flow column and Sephadex G 100 column in order. Con.1 was the constituent of high molecular weight and the other three were all of low molecular weight. Micro Kjeldahl analysis showed that Con.1 contained 3.95% of nitrogen and neither did the other three parts. The antisenile effects of the four parts of ABPS were studied with Drosophila melanogaster. Results showed that Con.1 has no antisenile effect and all the others could significantly increase the average body weight by 3.85%-5.47% and significantly prolonged the average lifespan by 2.61%-3.16%of D. melanogaster at the concentration of 2 or 5 mg/g (ABPS/medium).
牛膝多糖的组分分析及抗衰老活性研究
谈 锋 邓 君
（西南师范大学生命科学学院，重庆400715）
摘要：牛膝多糖(Achyranthes bidentata polysaccharides, ABPS)是苋科植物牛膝(Achyranthes bidentata Bl.)根中的水溶性多糖,先后用DEAE-Sepharose fast-flow柱和Sephadex G-100柱将牛膝粗多糖分为4个组分,分别命名为:Con.1、Con.2、Con.3和Con.4.Micro-Kjeldahl试验表明,Con.1中含有氮元素3.95%,另3个小分子组分不含氮元素.以果蝇为动物模型研究了该4个牛膝多糖组分的抗衰老作用,结果表明,大分子组分Con.1对果蝇无抗衰老作用,而另3个小分子量组分在培养基中浓度为2和5 mg/g(多糖质量/培养基质量)时,都可显著或极显著地使果蝇平均体重增加3.85%-5.47%, 并使果蝇平均寿命延长2.61%-3.16%.
关键词： 牛膝；多糖；DEAE-Sepharose 快速柱层析；Seohadex-G-100 柱层析；果蝇；抗衰老作用
全 文 ：