Using Lilium davidii Duchartre pollen as material, the calcium ion-fluorescence indicator fluo-3AM was loaded successfully into the pollen grains by low temperature loading method. Laser confocal scanning microscopy was used to study the effect of extracellular calmodulin on intracellular calcium. It is found that the purified exogenous calmodulin could elevate the intracellular calcium ion concentration, and the effect was correlated with the concentration of exogenous calmodulin to a certain extent. Cell membrane nonpermeable inhibitor of calmodulin, W 7-agarose, and the anti-serum of calmodulin could decrease the cytosolic calcium level. The results show that the endogenous extracellular calmodulin may play an important role in maintaining and increasing the cytosolic calcium level in pollen grain cell.
细胞外钙调素对百合花粉细胞内钙离子浓度的影响
尚忠林1,2 马力耕1 王学臣2 孙大业1*
（1.河北师范大学生命科学学院，石家庄050016；2. 农业部植物生理生化重点开放实验室，北京1000094）
摘要：以川百合 (Liliumdavidii Duchartre)花粉为材料 ,利用低温装载法在完整花粉粒中成功地装载了酯化形式的钙离子荧光指示剂fluo-3AM ,利用激光共聚焦显微技术研究了细胞外钙调素对细胞内游离钙离子浓度的影响。结果发现外源纯化钙调素可以使细胞内游离钙离子的浓度升高 ,在一定范围内促进效果与钙调素浓度呈正相关。不能透过细胞膜的钙调素拮抗剂W7-agarose和植物钙调素抗血清处理都可以使花粉细胞质中游离钙离子浓度降低 ,表明内源细胞外钙调素可能在维持和促进花粉细胞质中游离钙离子方面具有重要作用
关键词： 川百合；花粉；细胞外钙调素；游离钙离子；低温装载；激光共聚焦显微术
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