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Characteristics of CO2 Exchange and Chlorophyll Fluorescence of Transgenic Rice with C4 Genes


The responses of photosynthesis of phosphoenopyruvate carboxylase (PEPC), pyrurate dikinase (PPDK), NADP malic enzyme (NADP-ME) and PPDK+PEPC transgenicrice (Oryza saltiva L.) plant to light, temperature, CO2 and the characteristics of chlorophyll fluorescence under photoinhibition conditions were studied. The results were as follows: 1. The light saturated photosynthetic rates of transgenic rice plants were higher than that of wild type, in which the light saturated point of PEPC and PPDK+PEPC transgenic rice plants was 200 μmol·m-2·s-1 higher than that of untransformed rice and the light saturated photosynthetic rates were 51.6% and 58.5% respectively. The carboxylation efficiency of PEPC transgenic rice plant increased by 49.3% and the CO2 compensation point decreased by 26.2% than that of untransformed rice. Under high temperature (35 ℃), the photosynthetic rate of PEPC transgenic rice plant was higher over 17.5% than that of untransformed rice. 2. On the 8th day after photoinhibition treatment, the PSⅡ photochemical efficiency (Fv/Fm) and photochemical quenching (qP) of PEPC and PPDK+PEPC transgenic rice plants decreased by about 20%-30% while the non photochemical quenching(qN)increased by approximately 30%. But Fv/Fm and qP of untransformed rice decreased by over 50% while qN increased by less than 10%. The result suggested that transgenic rice plants were more tolerant to photoinhibition.


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