The evaluation on the callus embryogenesis capacity of 15 genotypes of citrus showed that stress treatments were conducive to somatic embryogenesis and could enhance the recovery of the missed capacity of embryogenesis for some genotypes. Randomly amplified polymorphic DNA (RAPD) and methylation sensitive amplified polymorphism (MSAP) analysis indicated that there existed significant differences in DNA methylation status between the callus capable of producing somatic embryoids and that which missed the embryogenesis capacity of the same genotype Newhall navel orange (Citrus sinensis Osb. cv. Newhall). The DNA methylation level of the former was lower than that of the latter. However, RAPD profiles did not show any difference between these two kinds of callus.
逆境处理和DNA 甲基化影响柑橘体细胞胚发生
郝玉金* 邓秀新**
（华中农业大学作物遗传改良国家重点实验室，武汉430070）
摘要： 对15种柑橘胚性愈伤组织进行体细胞胚诱导，发现逆境处理有利于体细胞胚发生，并可以恢复部分品种的体细胞胚发生能力。对具有和失去体细胞胚发生能力的两种纽荷尔脐橙（Citrus sinensis Osb.）愈伤组织进行随机扩增多态性-DNA（RAPD）分析没有检测到带型的差异，而对它们的甲基化敏感扩增多态性（MSAP）进行分析则发现两种愈伤组织间具有明显的DNA甲基化差异，具体细胞胚发生能力的愈伤组织的甲基化水平较失去体细胞胚发生能力的低。
关键词： 干燥处理；低温处理；DNA 甲基化；体细胞胚发生；RAPD; MSAP
通讯作者.E-mail:<dxxwwlj @ public.wh.hb.cn>.
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