Two cDNA libraries were constructed from microdissected 214 rice proembryos (2-3 d after pollination) and 121 just differentiating young embryos (3-5 d after pollination) respectively through RT-PCR technique. The primary libraries had a total of 3.7×10 phages for the proembryos and a total of 2.5×10 phages for the just differentiating young embryos, in which 96% of the phages were recombinants. Insert sizes ranging from 400 bp to 3?500 bp were obtained. All of theabove mentioned accorded with the general requirements of cDNA library construction.
水稻原胚和刚启动分化的幼胚cDNA文库的构建与分析
陈绍荣 李师弢 吕应堂 杨弘远*
（武汉大学生命科学学院发育生物学研究中心，武汉430072）
关键词： 水稻；原胚；分化胚；cDNA文库
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