The relationship between superoxide and singlet oxygen in PSⅡ particle of spinach under strong illumination was studied with spin-trap ESR technique. The generation of superoxide was increased under D2 environment which prolonged the half life of singlet oxygen. The generation of superoxide was decreased when histidine existed as a scavenger of singlet oxygen. It is highly possible that the superoxide generated in PSⅡ particle originates from singlet oxygen.
光系统II颗粒内单线态氧诱导产生超氧阴离子自由基
刘科1孙健1,2徐英凯1 陈中伟1 张启元1 张兴康1 匡廷云2 刘扬1*
（1. 中国科学院化学研究所，北京100080；2. 中国科学院植物研究所，北京100093）
关键词： 超氧阴离子自由基；单线态氧；PSII；活性氧；自旋捕捉-电子自旋共振技术
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