Abstract:The present paper reports that 0.17% fragrant, yellowish green colored volatile oil was obtained from Lysimachia foenumgraecum Hance by steam distil- lation with the following physical constants: nD20 1.4824, d1515 0.9059, [α]D25 -18.6 (c=1.1,CHCl3). 2. From the volatile oil a large amount of palmitic acid was separated and identified by its rap, elementary analysis and IR. 3. The volatile oil was divided into the acidic portion, the constituents in which were later transferred into their methyl esters (A), and the neutral and phenolic por- tion (B). (A) and (B) were analyzed by GC and GC-MS. The result of analysis showed that a total of 59 chemical constituents were indentified in the volatile oil; (A) con- sisted of 20 and (B) consisted of 39 constituents. 4. From the results as shown in the GC figures I and 2, hexahydrofarnesylacetone, being a rarely occured constituent in the natural product, and dibutyl phthalate might be considered to be the principal neutral costituents, just as the hexadecanoic acid and margaric acid to be the principal acidic constituents in the volatile oil of Lysimachia foenumgraecum Hance.