作 者 :WU Shi-An, Lü Hai-liang, YANG Ji, RAO Guang-Yuan, YOU Rui-Lin, GE Song
期 刊 :植物分类学报 2000年 2期
Keywords:Polygonatum, Systematics, RFLP, trnK gene, rpl16 gene,
Abstract:
Phylogenetic relationships in the tribe Polygonateae s. l. of Liliaceae were investigated
with an RFLP analysis of two PCR-amplified chloroplast genome DNA fragments. One fragment is
the transfer RNA gene for lysine( trnK gene) including the maturase-encoding gene matK, and the
other is the rpl16 gene which codes for a chloroplast ribosomal protein in large subunit. The trnK
gene is ca. 2600 bp in length in all the taxa, but the rpl16 gene ranges from ca. 1140 bp to ca.
1320 bp in length among different genera and in Polygonatum. The results suggested that the tribe
Polygonateae s. str. consisting of Polygonatum, Disporopsis, Smilacina and Maianthemum is
closely related to Convallaria in Convallarieae, and supported the earlier results that Streptopus and
Disporum should be removed out from the tribe Polygonateae s. l.. The results also showed that Po-
lygonatum formed a clad with Disporopsis, while Smilacina lumped with Maianthemum in the tribe
Polygonateae s. str.. In addition, the present study supports the view that S. ginfoshanicum
should be transferred from Smilacina to Polygonatum.