Abstract:Fruit fly species were collected through methyl eugenol-baited and cue-baited traps and from attacked fruits and melons in 2005 and 2006 at Ruili, Lujiangba, Liuku, Baoshan, and Dali in the western Yunnan. The species composition and diversity of fruit flies, and species similarity were studied, and cluster analysis was conducted. There were two occurrence patterns of the fruit fly species. One was year around occurrence at Ruili and Lujiangba, the other was seasonal occurrence at Liuku, Baoshan and Dali. The dominant species were Bactrocera dorsalis and B. tau in the five regions. The highest species richness of fruit flies was found at Ruili with 20 species, following at Lujiangba with 11 species. The lowest species richness was found at Dali with only 3 species. The lowest diversity index was found at Liuku, and the highest at Lujiangba. The similarity index of species ranged from 0.15 to 1.00. The results provided fundamental data for understanding distributions and diversity of fruit fly species and preventing from damage of the fruit flies in the western Yunnan, China.