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作 者 ：王霞,陈哲,袁红朝,吴敏娜,魏文学,吴金水,秦红灵*

期 刊 ：生态学报 2010年 30卷 14期 页码：3865~3874

关键词：16S rDNA；水稻土；长期施肥；细菌多样性；系统发育分析；

Keywords:16S rDNA, rice straw, bacterial diversity, phylogenetic analysis,

摘 要 ：利用中国科学院桃源农业生态试验站施肥制度长期定位试验田对照（CK）和稻草还田（OM）施肥处理的土壤样品，应用16S rDNA克隆文库技术直接提取土壤微生物总DNA，分别构建细菌16S rDNA克隆文库，并进行序列测定和分析。结果表明，与对照（CK）相比，稻草还田（OM）后土壤细菌群落结构发生了显著改变，土壤细菌多样性和均匀度均有所降低。对照（CK）和稻草还田（OM）两个施肥处理的优势种群均为变形菌，酸杆菌次之；稻草还田减少了变形菌、疣微菌、绿弯菌和绿菌的分布，而增加了硝化螺旋菌的分布。16S rDNA系统发育分析则表明，稻草还田对酸杆菌群落结构影响最大，其次是疣微菌和δ-变形菌。

Abstract:Paddy soil bacterial community structure was studied through 16S rDNA gene. The soil samples were collected from the long-term fertilization experiment field at the Taoyuan Agro-ecosystem Research Station. Total microbial DNA was directly extracted from both treatments including amended with rice straw (OM) and CK. The clone libraries were constructed after cloning and sequencing. The result showed that the bacterial communtiy composition of the paddy soil amended with rice straw (OM) was changed significantly compared with CK, both Shannon index and evenness decreased indicating its bacterial diversity was decreased. Proteobacteria were predominated both in rice straw (OM) and CK treatments, followed by Acidobacteria. Application of rice straw induced obvious decrease of the genotypes of Proteobacteria, Verrucomicrobia, Chloroflexi and Chlorobi compared to CK, in the contrast, the genotypes of Nitrospira increased. The phylogenetic tree based on the 16S rDNA sequences showed that application of rice straw varied the distribution of Acidobacteria greatest, followed by Verrucomicrobia and Delta-Proteobacteria. Therefore, application of rice straw had an important impact on bacterial community structure.

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