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作 者 ：蔡晓明，,孙晓玲，,董文霞，,王国昌，,陈宗懋*

期 刊 ：生态学报 2009年 29卷 1期 页码：169~177

关键词：zNoseTM；GC-MS；茶树；挥发物；茶尺蠖；机械损伤；

Keywords:zNoseTM, GC-MS, tea plant, volatiles, Ectropis obliqua Prout, mechanical damage,

摘 要 ：与传统的植物挥发物分析手段相比，zNoseTM具有采样时间短、快速分析、便于携带等不可比拟的优势。以茶树为研究对象，应用GC-MS、zNoseTM对茶尺蠖取食诱导的茶树挥发物进行了同步分析；并发挥zNoseTM采样时间短、快速分析的优势，研究了机械损伤茶树叶片后，4-甲基-3-戊烯醛、顺-3-己烯醇、顺-3-己烯醇醋酸酯等3种绿叶气味物质的释放规律。结果表明：GC-MS与zNoseTM对茶尺蠖取食诱导的茶树挥发物的定性分析结果差别不大；但两者测定茶尺蠖取食诱导的茶树挥发物中顺-3-己烯醇、苯乙腈、吲哚、反-石竹烯等4种组分的相对含量有一定差异。在此基础上，通过zNoseTM测定发现，无论磨损还是针扎损伤新叶，机械损伤的程度越大，3种绿叶气味物质的释放量越大；新、老叶片在遭受同样的机械损伤后，3种绿叶气味物质的释放不完全相同；新叶经针扎损伤后，3种绿叶气味物质释放高峰的出现时间不同。就zNoseTM在植物挥发物分析中的应用前景进行了讨论。

Abstract:zNoseTM possesses the following advantages including short sampling time, rapid analysis and portability compared with conventional analytical method. We analyzed sychronously the volatiles emitted from tea plants damaged by Ectropis obliqua Prout larvae with GC-MS and zNoseTM, and the emission of the green leaf volatiles（4-methyl-3-pentenal，（Z）-3-hexen-1-ol，(Z)-3-hexenly acetate） produced by tea leaves after mechanical damage were analyzed with zNoseTM. The qualitative analysis of the volatiles emitted from tea plants infested by Ectropis obliqua Prout with GC-MS or zNoseTM showed the similar results, however the relative contents of (Z)-3-hexen-1-ol，benzyl nitrile，indole，（E）-caryophyllene obtained from both showed a certain difference. A further investigation with by zNoseTM showed that more damage level resulted in the more emission of green leaf volatiles when young leaves were damaged mechanically with needle (sting) or abrasive paper (scratch); the emission of green leaf volatiles induced by the same degree mechanical damage from old leaves and young leaves was different; and the emission peak of 4-methyl-3-pentenal，（Z）-3-hexen-1-ol and (Z)-3-hexenyl acetate appeared at different time after young leaves were stung. Finally, the prospect on the application of zNoseTM in plant volatiles analysis were discussed.

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