Abstract:It′s difficult to restore vegetation in limestone area, due to the severe and thin soil layer of the limestone habitat. However, some plants adaptive to limestone soil like Broussonetia papyrifera grow in this habitat, the reason maybe that these plants possess rhizospher microbes like Vesicular-arbuscular mycorrhizal fungus. In this paper, an experiment was conducted on Broussonetia papyrifera seedling by disposals of single inoculation , co-inoculation and non-inoculation using three mycorrhizal fungus，which are Glomus.mosseea, Glomus.versiforme and Glomus.diaphanum. Some physiological indexes of leaves are measured to study the metabolism response in this experiment after 3 months. The results show that the concentrations of soluble sugar, protein , proline and chlorophyl a and b are significantly increase in the leaves of the host plants in inoculation group compared with none-inoculation. However, the malondialdehyde concentration decrease or remain equivalently in inoculation disposal. All the substances have certain correlation at physiological metabolism. Even as metabolite, there are different physiologically response in different inoculation disposals by AMF. The interactional selection exist in host plants and AMF. It could be conclude that inoculation AMF could enhance the stress tolerance of Broussonetia papyrifera seedling growing in limestone habitat.