摘 要 ：选用分离自河北、内蒙古等5省区的岩黄芪根瘤菌30株及17株已知参比菌株,进行了营养利用、抗生素抗性、耐逆性及生理生化反应等12 5项表型性状研究,所得数值分类树状图表明不同宿主及不同地域的岩黄芪根瘤菌的表型多样性。通过对其中部分菌株进行16 S r DNA PCR- RFL P及BOX- PCR指纹图谱分析,聚类结果表明供试岩黄芪根瘤菌具有遗传多样性。
Abstract:The legume species within the genus Hedysarum are deep-rooted annual or perennial herbs, and they are well distributed in the temperate zones. About 40 species of Hedysarum are native to China and they are potential foliages and are used as resources of herbal medicine. These legumes are pioneers plants, but its geographic distribution may be determined by the existence of proper rhizobia. Aiming at understanding the diversity of rhizobia associated with them, we isolated thirty rhizobial strains from root ...