Abstract:A micro-plot experiment in field was conducted with15N 160 kg·hm-2total amounts by three kinds of treatments of disposable bottom application nitrogen before sowing (T1),55% nitrogen before sowing and 45% nitrogen during seedling stage(T2), and 55% nitrogen before sowing, 30% nitrogen during seedling stage and 15% nitrogen applied during squaring stage (T3) to investigate the use efficiency and fate of fertilizer nitrogen using the potato cultivar Favorite. Results showed that almost 46% to 52% of total N uptake by potato came from fertilizer, and 48% to 54% N derived from soil and seed-tuber. The nitrogen use efficiency ranged from 35.16% to 39.99%, with15N-fertilizer residue of 47.71% to 51.78%, and15N-fertilizer loss of 8.23% to 16.98% among three treatments.15N-fertilizer residual mainly distributed in 0 to 15cm top soil under the three types of nitrogen application. The amounts15N-fertilizer residue in 0 to 15cm top soil showed a rising trend with delaying nitrogen application, but dropped in 15 to 45cm soil profile. There was no significant effect of nitrogen application types on accumulation of total dry matter and tuber dry matter of potato, but15N-fertilizer utilization ratio and residue ratio of T3 were significantly higher than T1 and T2. These results indicated that T3 was the suitable nitrogen application types in the view of economic and environmental benefit. And the research provides guidance basis on effective management on nitrogen nutrient of potato.