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作 者 ：董万鹏, 龙秀琴, 代丽华, 孙立坤, 李培林, 杨雨嘉

期 刊 ：核农学报 2016年 30卷 8期 页码：1656-1663

关键词：西番莲；冷驯化；低温半致死温度；抗寒性；越冬表现；

Keywords:Passiflora edulis, cold acclimation, semi-lethal low temperature, cold resistance, wintering performance,

摘 要 ：为探明越冬期间各株系西番莲低温半致死温度(LT₅₀)的变化情况,以7个株系西番莲枝条为研究对象,测定其越冬期间的相对电导率,配合Logistic回归方程计算LT₅₀,研究LT₅₀下处理时间对相对电导率、MDA与抗坏血酸(Vc)含量的影响,同时进行寒害调查并观测各株系西番莲的越冬表现。结果表明,越冬期间,各株系西番莲的LT₅₀均明显降低,在冷驯化终了期其对应的LT₅₀分别为:平塘1号(-3.59℃)、紫香1号(-2.51℃)、版纳9号(-1.96℃)、版纳10号(-1.95℃)、版纳4号(-1.02℃)、榕江1号(-1.82℃)、金沙1号(-1.83℃)。西番莲芽梢、叶片、1年生枝条、2年生木质化枝条的LT₅₀分别为:-1.16℃、-1.39℃、-2.83℃、-3.11℃,不同部位对低温敏感性差异显著。各株系西番莲越冬表现情况与LT₅₀测定结果较吻合,平塘1号越冬表现最佳,采取防寒措施后越冬存活率达100%。西番莲枝条的相对电导率、MDA含量随LT₅₀下处理时间的增加而增加,5~15h期间增幅最大,而Vc含量则先增后降,并在5~15h期间含量较高,说明低温胁迫对西番莲枝条的伤害主要发生在 5~15h期间。本研究为选育抗寒性强的西番莲优良品种提供了理论依据。

Abstract:In order to explore the changes of LT50 for varieties of P. edulis during overwintering period, seven varieties of P. edulis were employed to measure the relative electric conductivity of P. edulis. Analyzing the effects of LT₅₀ processing time on relative electric conductivity, the content of MDA and ascorbic acid (Vc) by logistic regression, meanwhile, observing their chilling injury during overwintering. Results indicates that the LT50 has declined clearly during the winter period and ranked as follows: Pingtang No.1(-3.59℃), Zixiang No.1(-2.51℃), Banna No.9(-1.96℃), Banna No.10(-1.95℃), Banna No.4(-1.02℃), Rongjiang No.1(-1.82℃) and Jinsha No.1(-1.83℃). Different parts of P. edulis have different LT₅₀, mud tip(-1.16℃), leaves (-1.39℃), annual branches(-2.83℃) and biennial branches(-3.11℃), indicating significant differences in LT50 for various parts. The observations and experimental results have reached the same results that Pingtang No.1 is the best for surviving in winter, and the wintering survival rate was 100% by taking proper cold-resistant measures. With the increasing of LT₅₀ processing time, relative electric conductivity and MDA content increased as well, experiencing max increases during 5 to 15 hours. However, the Vc content was increasing first then decreasing and had the highest content during the period of 5 to 15 hours, which proved that the most injury effects of low temperature for P. edulis branches occurred during 5 to 15 hours. This research provides theoretical basis for breeding varieties of P. edulis with the strong cold resistance.

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