作 者 ：李娟， 安锋， 林位夫， 周立军， 陈俊明， 潘剑， 黄坚雄， 郑定华
期 刊 ：核农学报 2016年 30卷 1期 页码：193-200
关键词：五指毛桃；#x0201c；3414#x0201d；肥料试验；成龄胶园间作；SPAD值；叶绿素含量；
Keywords:Wuzhimaotao, fertilizer experiments, intercropping of mature rubber plantation, SPAD value, chlorophyll content,
摘 要 ：为探讨在“3414”肥料试验中叶绿素仪测定叶绿素的精确度,以及不同施肥处理对五指毛桃叶片SPAD 值与叶绿素含量的影响,采用SPAD叶绿素仪和酒精-丙酮浸提法对五指毛桃不同叶片的SPAD值、叶绿素a、叶绿素b 和总叶绿素含量进行测定。结果表明,不同施肥处理对五指毛桃3片叶片的SPAD值,倒1叶的叶绿素a、叶绿素b和总叶绿素含量,倒2叶的叶绿素a以及倒3叶的叶绿素b、总叶绿素含量的影响不显著;对倒2叶的叶绿素b、总叶绿素含量,倒3叶的叶绿素a影响显著。氮、磷、钾肥对五指毛桃叶片SPAD值和叶绿素含量效应依次为N 肥>P 肥>K 肥。SPAD值、叶绿素a、叶绿素b及总叶绿素含量从倒1叶至倒3叶呈现逐渐增加的趋势,但增加的幅度因施肥处理不同而不同。倒1叶的SPAD值与叶绿素a、叶绿素b及叶绿素总量呈负相关;倒2叶的SPAD值与叶绿素a、叶绿素b及叶绿素总量均呈极显著正相关;倒3叶的SPAD值与叶绿素a呈负相关,而与叶绿素b及叶绿素总量均呈显著正相关,表明在“3414”肥料试验中倒2叶的SPAD值更能准确地预测其叶绿素的绝对含量。该研究为叶绿素仪在五指毛桃生理实验中正确应用提供了科学依据,也为五指毛桃在成龄胶园间作实践提供了理论指导。
Abstract:To investigate the accuracy of SPAD chlorophyll meter in chlorophyll measurements and the effect of different fertilization treatments on SPAD value and chlorophyll content of Ficus hirta Vahl. (Wuzhimaotao) in ‘3414’ fertilizer experiment. SPAD value, chlorophyll a, chlorophyll b and total chlorophyll contents of different F. hirta leaf position were determined with both SPAD chlorophyll meter and alcohol-acetone extraction methods. Results showed that fertilization treatments showed no significant influence on SPAD values of three leaves chlorophyll a, chlorophyll b and total chlorophyll contents of the first-top-leaf, as well as chlorophyll a and total chlorophyll contents of the second-top-leaf, and chlorophyll b and total chlorophyll content of the third-top-leaf. However, fertilization treatments significantly changed the chlorophyll b and total chlorophyll contents of the second-top -leaf, and the chlorophyll a content of the third-top-leaf. The effects of nitrogen, phosphorus and potassium applications on the SPAD value and chlorophyll contents was in the following order: N fertilizer> P fertilizer> K fertilizer. SPAD value, chlorophyll a, chlorophyll b and total chlorophyll contents, increased, gradually, from the first-top-leaf to the third-top-leaf. There were negative correlations between SPAD value and chlorophyll a, chlorophyll b and total chlorophyll contents, respectively, for the first-top-leaf, and negative correlations between SPAD value and chlorophyll a content of the third-top-leaf. However, significant positive correlations between SPAD value and chlorophyll a, chlorophyll b and total chlorophyll contents, respectively, were identified for the second-top-leaf. Similarly, significant positive correlations between SPAD value and chlorophyll b content and total chlorophyll content were found in the third-top-leaf of F. hirta. All these results suggest that the SPAD value of the second-top-leaf is the best estimation for the chlorophyll contents of F. hirta in the “3414” fertilizer experiments. These findings provide a scientific basis for using chlorophyll meter correctly in the physiological experiment of F. hirta, and provide some theoretical guiding for the intercropping of F. hirta with mature rubber.