Abstract:To discuss the effect of additives and their additive amount on the gel properties of myofibrillar protein from Dosidicus gigas muscle, a response model for hardness of the gel and four kinds of additives (chitosan, TGase, carrageenan and sodium alginate) was established using response surface analysis methodology based on the single factor experiment. The results indicated that the optimal proportion of four kinds of additives was chitosan 0.40%, TGase 0.50%, carrageenan 0.90% and sodium alginate 0.25%, respectively. Under the optimal condition, the hardness of myofibrillar protein gel reached the maximum of 859.85 g with the WHC of 96.34%. In conclusion, the compound additive has a significant improvement on the gel properties of myofibrillar protein from Dosidicus gigas muscle, which can provide the theory basis for production of high-quality gel products using Dosidicus gigas as raw materials.