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作 者 ：李玉萍, 罗凤霞, 汤庚国, 史慧梅

期 刊 ：核农学报 2016年 30卷 4期 页码：676-684

关键词：兰花；杂交育种；SRAP；F1鉴定；

Keywords:Cymbidium, cross breeding, SRAP, determination of F1,

摘 要 ：为了更好地在兰花育种中判别真杂种,采用SRAP分子标记技术对春兰紫萼×大花蕙兰日本绿的10个杂种F₁株系进行杂种真实性鉴定。结果表明,21对多态性较高的随机引物共扩增得到364条带,多态性条带达307条,占总带数的84.34%,试验所选10个杂种F₁株系均为真实杂种,且与母本相似性较高。聚类分析表明,SRAP分子标记技术可作为兰花杂交育种早期杂交种筛选鉴定的有效方法。因此,利用SRAP分子标记技术可为兰花育种筛选提供理论依据,加快兰花育种进程。

Abstract:In order to distinguish the hybrid in orchid cross breeding, SRAP molecular markers were used to identify hybrid reliability of 10 hybrid lines among Cymbidium goeringii Zi‘e× C. hybridum Riben LYu. The results suggested that 364 DNA bands were obtained with 21 pairs of highly polymorphic random primers. Among these DNA bands amplified, 307 (84.34%) were polymorphic. Hybrid Identification results showed that the selected 10 hybrid F1 were true hybrids with high similarity to their mothers. Clustering analysis showed that SRAP molecular markers are effective in identifying and screening early hybrids in the orchid cross breeding. Therefore, using SRAP molecular marker technology can provide a theoretical basis for screening orchid hybrid and orchid accelerate the breeding process.

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