---

期 刊 ：核农学报 2009年 23卷 6期 页码：958-963

关键词：陆地棉；棉纤维；开花后天数(DPA)；RT-PCR；

Keywords:Gossypium hirsutum L, cotton fiber, Day post-anthesis (DPA), RT-PCR,

摘 要 ：以陆地棉（Gossypium hirsutum L.）高代品系CJ01在开花后5、10、15、20、25d（DPA）共5个不同发育时期的棉花纤维为材料，分别利用常规半定量RT-PCR法、QuantumRNA™18S内标相对定量法以及实时荧光定量 RT-PCR法，对棉花肉桂醇脱氢酶（GhCAD）基因在棉花纤维不同发育时期中的表达进行相对定量分析，并测定了内标表达的稳定性。结果表明3种方法获得相似的结果，GhCAD基因在5～15DPA时表达量较低，20、25 DPA时表达量升高。3种方法虽各有利弊，但均为研究基因量化表达的有力工具。

Abstract:In this study, cotton fibers from 5, 10, 15, 20, 25 days post-anthesis (DPA) of strain CJ01 in upland cotton (Gossypium hirsutum L.) were used as materials. The relative quantitative expression of GhCAD gene at different developmental stages of cotton fiber were analyzed by conventional semi-quantitative RT-PCR, QuantumRNA ™ 18S internal standard relative quantitative RT-PCR and real-time RT-PCR respectively. The stability of internal standard expression was also tested. Results showed that different methods obtained similar results: the relative quantitative expression of GhCAD was lower during 5～15 DPA and increased during 20～25 DPA in cotton fiber. Though three methods have their advantages and disadvantages, all of them are useful tools for testing of gene relative quantitative expression.

---