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作 者 ：杜虹，庄东红，黄文华

期 刊 ：热带亚热带植物学报 2000年 8卷 2期 页码：109~112

关键词：大白菜；子叶；离体培养；AgNO-3；芽再生；

Keywords:Brassica campestris, Cotyledon, In vitro culture, AgNO3, Shoot regeneration,

摘 要 ：以大白菜"O2号杂交早皇白"无菌苗的子叶为材料,用附加BA 2.O mg L^-1、NAA 0.1-1.0 mg L^-1的MS培养基培养,能直接诱导分化出芽.最适激素比例为BA 2.O mg L^-1、NAA 0.5 mg L^-1,芽的分化率为31.6%。在上述培养基里添加2 mg L^-1的AgNO₃能使芽的再生频率提高至86.5%。

Abstract:A tissue culture system for obtaining high frequency shoot regeneration of Brassica campestris was studied. Shoots could be induced directly from cotyledons cultured on MS medium supplemented with 2.0 mg L^-1 BA and 0.5 mg L^-1 NAA, the shoot regeneration rate being 31.6%. Addition of 2.0 mg L^-1 AgNO₃ in the medium could greatly increase the frequency of shoot regeneration, and the shoot regeneration rate reached to 86.5%.

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