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作 者 ：宫伟娜,万方浩,谢丙炎,郭建英,周艳

期 刊 ：热带亚热带植物学报 2009年 17卷 5期 页码：451~457

关键词：紫茎泽兰；热激蛋白基因；启动子；克隆；染色体步行；TAIL-PCR；

Keywords:Ageratina adenophora, hsp gene, Promoter, Clone, Chromosome walking, TAIL-PCR,

摘 要 ：以入侵植物紫茎泽兰(Ageratina adenophora Sprengel)为材料，采用基于PCR方法的染色体步行和改良的热不对称交错PCR(thermal asymmetric interlaced PCR，TAIL-PCR)两种方法分别克隆到hsp90和hsp17.66基因的5’上游启动子序列，长度分别为864 bp和1485 bp(GenBank登录号分别为FJ434253和FJ434252)。测序结果表明：这两个启动子序列均具有hsp启动子特有的HSE元件，及其他一些启动子顺式作用元件, 如TATA-box, CAAT-box等。

Abstract:The promoter region of hsp90 with 864 bp (GenBank No. FJ434253), from invasive weed Ageratina adenophora was amplified based on PCR methods, which are available as chromosome walking from a known sequence to an unknown region. Moreover, another promoter region of hsp17.66 with 1 485 bp (GenBank No. FJ434252) was cloned by thermal asymmetric interlaced PCR (TAIL-PCR). Both of the 5′-flanking regions of hsp90 and hsp17.66 contained specific HSE element and several cis-acting elements, such as TATA-box, CAAT-box, et al.

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