摘 要 ：以谭清苏铁(Cycas tanqingii D. Y. Wang)雌雄植株半年生羽叶为材料，用优化的CTAB法分别提取其全基因组DNA，进行RAPD单因子梯度实验和正交实验以优化扩增条件。应用160个RAPD随机引物检测基因组DNA，雌雄植株均扩增出1 450多条带，其中引物S0465扩增出与谭清苏铁雌株高度相关的RAPD标记(TQS465-500)，其大小约为500 bp，该标记与雄株没有关联。
Abstract:The total genomic DNA of the male and female cycads (Cycas tanqingii D.Y. Wang) was extracted from the leaflets of half a year old using an optimized CTAB protocol. The RAPD-PCR amplification conditions were optimized by monofactor and multifactor orthogonal experiments. A total of 160 primers were used for RAPD-PCR amplification and more than 1 450 fragments were obtained from the male or the female plants. A fragment of about 500 bp only linked to the female was generated steadily by the S0465 (CCCCGGTAAC) primer, which can be used in sex determination of C. tanqingii.