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作 者 ：洪森荣;尹明华*;王艾平

期 刊 ：植物研究 2013年 33卷 6期 页码：738-745

关键词：江西铅山红芽芋；脱毒苗；球茎；愈伤组织；再生体系；

Keywords:Jiangxi Yanshan red bud taro(Colocasia esculenta L. Schott var. cormosus CV. Hongyayu), virusfree plantlet, corm, callus, regeneration system,

摘 要 ：以江西铅山红芽芋脱毒苗为试材，研究不同因素对红芽芋脱毒苗球茎愈伤组织诱导及其再生体系的影响，以期对红芽芋脱毒苗的再生体系进行优化。结果表明，红芽芋脱毒苗球茎愈伤组织诱导的最佳培养基是MS+TDZ 2 mg·L^-1+2,4-D 1 mg·L^-1。红芽芋脱毒苗球茎愈伤组织分化的最佳培养基是MS+TDZ 2 mg·L^-1+NAA 1 mg·L^-1。红芽芋脱毒苗不定芽生根的最佳培养基是1/2MS+NAA 0.5 mg·L^-1+PP₃₃₃ 0.5 mg·L^-1。红芽芋再生苗最好的移栽基质为发酵后的腐锯木屑。红芽芋脱毒苗球茎愈伤组织再生苗移栽时最佳的PP₃₃₃浓度为20～50 mg·L^-1。本试验成功建立了红芽芋脱毒苗球茎愈伤组织的再生体系，为红芽芋脱毒苗转基因的研究和种质创新奠定了基础。

Abstract:The effects of different factors on callus induction and its regeneration system were studied by using Jiangxi Yanshan red bud taro (Colocasia esculenta L. Schott var. cormosus CV. Hongyayu) virus-free plantlet corms as test materials. The results showed: the best culture medium of callus induction of red bud taro virus-free plantlet corms was MS+TDZ 2 mg·L^-1+2,4-D 1 mg·L^-1; The best culture medium of callus differentiation of red bud taro virus-free plantlet corms was MS+TDZ 2 mg·L^-1+NAA 1 mg·L^-1; The best rooting medium of red bud taro virus-free plantlet adventitious buds was 1/2MS+NAA 0.1 mg·L^-1+PP₃₃₃ 0.1 mg·L^-1. The best transplanting matrix of plantlets regenerated from red bud taro virus-free plantlet corm calli was rotted sawdust after fermentation; The best PP₃₃₃ concentration of plantlets regenerated from red bud taro virus-free plantlet corm calli for transplanting was 20-50 mg·L^-1. The regeneration system from red bud taro virus-free plantlet corms was successfully established, which establishs foundation for transgenic technology and germplasm innovation of Jiangxi Yanshan red bud taro virus-free plantlets.

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