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作 者 ：尹明华,洪森荣

期 刊 ：中草药 2009年

关键词：黄独；脱毒苗；快繁技术；优化；

Keywords:Dioscorea bulbifera L, .virus-free plantlets, rapid propagation system, optimization,

摘 要 ：目的以黄独脱毒苗为试材,研究不同因素对黄独带芽茎段芽增殖和生根的影响,以期对黄独脱毒苗的快繁技术进行优化。方法采用植物组织培养的方法进行茎尖培养和快繁研究,采用RT-PCR法对茎尖脱毒植株进行病毒检测。结果黄独脱毒苗带芽茎段的最佳培养基是MS+KT 2 mg/L+6-BA 1 mg/L+NAA 0.5 mg/L;黄独脱毒苗带芽茎段增殖的最佳蔗糖质量浓度和琼脂质量浓度分别是30和0 g/L;黄独脱毒苗带芽茎段生根的最佳培养基是1/2 MS+IBA 0.1 mg/L+NAA 0.5 mg/L+PP₃₃₃1 mg/L;黄独试管苗移栽的最好基质是珍珠岩:蛭石（2:1）;黄独试管苗移栽时最佳的PP₃₃₃质量浓度是50 mg/L。结论首次成功建立了黄独脱毒苗的快繁技术,为黄独脱毒苗的工厂化生产奠定了技术基础。

Abstract:Objective Effects of different factors on proliferation and rooting of stems with a bud were studied by using Dioscorea bulbifera as test material to optimize the rapid propagation system of D.bulbifera virus-free plantlets.Methods Plant tissue culture method was used in shoot tip culture and rapid propagation study,and RT-PCR method was used in virus detection of virus-free plantlets.Results The best proliferation medium of D.bulbifera stems with a bud was MS+KT 2 mg/L+6-BA 1 mg/L+NAA 0.5 mg/L;The best sucrose and agar concentration of D.bulbifera stems with a bud was 30 g/L and 0 g/L,respectively;The best rooting medium of D.bulbifera stems with a bud was 1/2 MS+IBA 0.1 mg/L+NAA 0.5 mg/L+PP_(333) 1 mg/L;The best transplanting matrix of regeneration plantlets from D. bulbifera stems with a bud was perlite-vermiculite(2:1);The best PP_(333) concentration of D.bulbifera regeneration plantlets for transplanting was 50 mg/L.Conclusion The rapid propagation system of D. bulbifera virus-free plantlets is established successfully for the first time,which provides a technological basis for factory production of D.bulbifera virus-free plantlets.

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