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作 者 ：胡盼1；仲崇禄1；张勇1；姜清彬1；陈羽1；陈珍1；韩强1；Khongsak Pinyopusarerk2；Didier Bogusz3

期 刊 ：植物研究 2016年 36卷 1期 页码：116-122

关键词：木麻黄；EST；SSR；

Keywords:Casuarinaceae, EST, SSR,

摘 要 ：从NCBI的EST数据库中获得的木麻黄EST序列共有34 752条，进行拼接后得到全长7 278.578 kb的非冗余序列(Unigene)12 062条，并从中检索得到分布于353条Unigene的367个SSR位点，SSR检出率为2.93%，平均分布距离为19.83 kb，包括39种重复基序类型。其中，以二核苷酸和三核苷酸为主要类型，在总SSRs中所占比例分别为57.77%和34.60%；而二核苷酸重复基序中，AG/CT所占比例最高，为93.87%；在三核苷酸重复基序中AAG/CTT所占比例最高，为44.09%。对检索出的EST-SSR位点设计得到97对引物，其中32对为可有效扩增引物。Blastx分析发现77.3%的含SSR位点的EST序列与非冗余蛋白序列数据库中功能序列具有同源性，而功能已知的序列中葡萄来源的序列占有最大比例（10.4%）。GO功能分类发现，含有SSR位点的EST序列中有47.3%至少具有1个GO注释，归入细胞组分的序列最多，而其中细胞质和细胞核的功能项所占比例较大。

Abstract:There are 34 752 ESTs of Casuarinaceae in the database of NCBI, resulting in 12 062 non-redundant sequences(Unigene) with total length of 7 278.578 kb. 367 SSR were discovered to distribution on 353 Unigenes, the frequency and repeat motif types number of these EST-SSRs was 2.93% and 39, and the mean distance was 19.83 kb. Dinucleotide and trinucleotide repeats were the dominant types among the EST-SSRs, accounting for 57.77% and 34.06%, respectively. AG/CT(93.87%) and AAG/CTT(44.09%) were the most abundant motifs for dinucleotide and trinucleotide. There were 97 pairs of primers designed for the obtained EST-SSR, and 32 of all could be effectively amplified. By Blast analysis, 77.3% ESTs sequences containing SSR locus were homologous with functional sequences in non-redundant protein sequences databases. The homology with functions from Vitis vinifera had the greatest proportion(10.4%). By GO functional classification, 47.3% EST sequences containing SSR locus had at least one GO ontology, the sequences relevant to cellular components were most, and the functional items relevant to cytoplasm and nucleus were highest.

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