---

期 刊 ：植物研究 2015年 35卷 3期 页码：471-475

关键词：微粉；超临界；理化性质；白藜芦醇；溶出度；抗氧化能力；

Keywords:ultrafine powder, supercritical antisolvent, micron particl, resveratrol, dissolution rate, antioxidant capacity,

摘 要 ：实验利用超临界SAS法使白藜芦醇在超临界状态下反溶剂重结晶，来制备白藜芦醇微粉借助超临界反溶剂法（SAS）来减小白藜芦醇的粒径从而提高其抗氧化能力。制备的条件为:反应温度为50℃，反应压力为20 MPa，白藜芦醇乙醇溶液的浓度为9 mg·mL^-1，给药速度为1 mL·min^-1。利用激光粒度仪和扫描电镜检测白藜芦醇微粉，结果显示白藜芦醇粉体较原粉粒径大大降低，平均粒径为0.68±0.03 μm。而后通过了傅里叶红外光谱、X衍射仪、高效液相色谱和质谱检测白藜芦醇微粉和原粉的各项特性。结果显示SAS过程并没有改变白藜芦醇的化学结构，但白藜芦醇微粉的结晶度大大降低，白藜芦醇的纯度也从原粉的98.5%纯化为微粉的99.2%。利用果蝇抗衰老实验来检测白藜芦醇的抗氧化能力。结果表明在白藜芦醇溶液浓度为，2.5、5和10 mg·mL^-1时，白藜芦醇微粉的抗氧化能力均高于原粉和空白实验。这说明白藜芦醇的不但具有抗氧化能力，而且白藜芦醇微粉在效果上优于白藜芦醇原粉。这为提高白藜芦醇品质提供了依据。

Abstract:By supercritical SAS, we made the resveratrol anti solvent recrystallization to prepare ultrafine resveratrol powder under supercritical condition. The conditions were under 50℃, 20 MPa, resveratrol ethanol solution concentration of 9 mg·mL^-1, and sample solution flow rate of 1 mL·min^-1. We analyzed the particles by Dynamic Light Scattering(DLS) equipment and Scanning Electron Microscopy(SEM). The particle size of the particles was greatly reduced and the average particle size was 0.68±0.03 μm. In addition, characterization of ultrafine Res and raw-resveratrol were tested by Fourier transform infrared spectroscopy(FT-IR), X-ray diffraction(XRD), High performance liquid chromatography(HPLC) and Liquid chromatography-mass spectrometry(LC-MS). SAS process didn‘t change the chemical structure of Res. The chemical structure of resveratrol was unaffected by the SAS process. The degree of crystallinity of the u-Res particles greatly reduced. We used the purity of the u-Res particles increased from 98.5% to 99.2% during the SAS process. Using drosophila anti-aging experiments to detect the oxidation resistance of resveratrol. The antioxidant capacity of resveratrol powder were higher than that in the second-stage and blank experiment under the concentration of resveratrol solution, 2.5, 5 and 10 mg·mL^-1. The resveratrol had the oxidation resistance, and ultrafine resveratrol powder was better than raw-resveratrol powder.

---