Abstract:Budding stems of Vaccinium corymbosum hybrid were used as the test materials to systematically investigate tissue culture. Proliferation and induction of cluster buds, effects on cluster buds induction and proliferation of the subculture time, test-tube rooting, ex vitro rooting, transplantation survival rate with these two different rootings in V.corymbosum hybrid were researched with tissue culture. The optimal proliferated and induced medium for V.corymbosum hybrid was WPM medium with 2.0 mg·L-1 ZT, and proliferation rate can reached 3.5; the proliferation rate can reached 24.00 with six times of subculture culture. The optimal medium for test-tube rooting was WPM medium with 0.5 mg·L-1 ZT, 0.1 mg·L-1 IBA with rooting rates of 80.73%±3.17%, and the rooting culture time is 100 d. The best methods for ex vitro rooting was taking explants diping in solution with 25 mg·L-1 IBA for 10s, and the nutrient solution is 1/6 WPM with the perlite as support materials with rooting rates of 80.00%±5.00%, and the rooting culture time is 40 d. The survival rate of ex vitro rooting is two times to test-tube rooting. The rapid propagation technique system have established and laid the technical foundation on industrialized seedling.