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作 者 ：罗猛1,2；宋卓悦1,2；胡娇阳1,2；牟璠松1,2*；乔琪1,2；祖元刚1,2

期 刊 ：植物研究 2015年 35卷 4期 页码：632-637

关键词：山里红叶；超声提取；总黄酮；Box-Behnken设计；抗氧化活性；

Keywords:Crataegus pinnatifida leaves, ultrasonic-assisted extraction, total flavonoids, Box-Behnken design, antioxidant activity,

摘 要 ：采用超声辅助提取法从山里红叶中提取总黄酮。通过BoxBehnken实验设计(BBD)结合响应面法(RSM)来优化超声提取的条件。影响山里红叶总黄酮提取效率的4个主要变量为液固比，温度，乙醇浓度和时间，得到的最佳值分别为15，40℃，40%，32 min。在此条件下，总黄酮的产率为15.50 mg·g^-1。体外抗氧化实验表明，山里红叶提取物的DPPH自由基清除能力为0.69 mg·mL^-1(以IC₅₀值表示) ，与传统的浸渍提取和热回流提取方法相比，超声提取的方法具有更好的抗氧化活性。实验结果表明超声提取法适用于提取山里红叶中的总黄酮，并且其提取物具有较好的抗氧化活性。

Abstract:We used ultrasonic-assisted extraction(UAE) to extract the total flavonoids from Crataegus pinnatifida leaves, and used Box-Behnken design(BBD) combined with response surface methodology(RSM) to optimize extraction conditions. We determined the effects of four variables in terms of ratio of liquid/solid, temperature, ethanol concentration and time on the total flavonoids contents(TFC). The optimized extraction parameters obtained the highest total flavonoids yield were under the ratio of liquid/solid of 15, 40℃, and 40% of ethanol for 32 min. Under these conditions, the yield was 15.50 mg·g^-1. By in vitro antioxidant assays, the extract exhibited a strong DPPH radical-scavenging in ability with IC₅₀ value of 0.69 mg·mL^-1. Compared with conventional maceration extraction(ME) and heat-refluxing extraction(HE) methods, UAE method showed remarkable advantages in terms of better antioxidant activities. Overall, the present results indicated that UAE was a suitable method for the extraction of total flavonoids from C.pinnatifida leaves and its extracts were potential antioxidant ingredients.

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