Abstract:ISSR markers were used to analyze the genetic diversity of 48 Brassica campestris cultivars. A total of 103 markers were scored from 9 primers, which were screened out from 60 random primers and demonstrated highly reproducible, clear and polymorphic bands. Each primer produced 11.4 bands. Among the obtained markers, 85 bands were polymorphic, accounting for 82.68% of the total. The genetic similarity coefficients among the cultivars ranged from 0.59 to 0.97, reflecting the high genetic diversity of B.campestris germplasms by ISSR markers. Cluster analysis with UPGMA method showed that these 48 germplasms could be divided into four groups, which were closely related to the leaf color of the different cultivars. The results will provide the reference to the study and usage of B.campestris germplasms.