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作 者 ：李树春1；夏辉2；严冬2；孟丙南3；姚立新3；刘婷婷4；崔永洪5；程玉飞6；赵曦阳2,5*

期 刊 ：植物研究 2015年 35卷 1期 页码：68-76

关键词：杨树；SSR；遗传距离；聚类分析；

Keywords:Populus spp., Simple Sequence Repeat(SSR), genetic distance(GD), cluster analysis,

摘 要 ：利用SSR分子标记对不同派别的48个杨树无性系进行遗传分析，计算不同无性系间的遗传距离。结果表明：16对引物共检测到81个多态性位点（从100~600 bp），不同无性系间的分子遗传距离在0.061 7~0.530 9，平均距离为0.250 4。派内种间遗传距离平均值变化范围为0.148 2~0.274 1，派间遗传距离平均值变化范围为0.330 2~0.401 2。聚类分析结果表明在聚类分析自检举值为0.70时，可以清晰的将48个无性系分成白杨派（Leuce）、黑杨派（Aigeiros）、青杨派（Tacamahaca）、大叶杨派（Leucoides）和胡杨派（Turanga）等5类。本研究利用分子手段检测不同派别杨树的遗传距离，为杨树杂交育种、分子育种等提供理论依据。

Abstract:Simple Sequence Repeat (SSR) was employed to study the genetic analysis and genetic distance (GD) among 48 clones in Populus L. Sixteen polymorphism markers produced 81 alleles (size ranged from 100-600 bp) totally. GD ranged from 0.061 7-0.530 9 among 48 poplar clones with the average of 0.250 4. GD ranged from 0.148 2-0.274 1 between inter-section and from 0.330 2-0.401 2 between different sections. By cluster analysis, 48 clones were clustered into 5 groups at 0.70, of which the clones divided into 5 section (sect.Leuce; sect.Aigeiros; sect.Tacamahaca; sect.Leucoides and sect.Turanga) clearly. Genetic distance of different section of Populus by molecular method can provide theoretical basis for cross breeding and molecular breeding in poplar.

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