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作 者 ：高畅;程大海*;高欣;赵尔丰

期 刊 ：植物研究 2010年 30卷 2期 页码：253-256

关键词：蓝莓果渣；纤维素酶；超声波；总酚；抗氧化；没食子酸；抗坏血酸；

Keywords:blueberry pomace, cellulase, ultrasonic, total phenolic, antioxidant activity, GAE,

摘 要 ：研究了蓝莓果渣提取物总酚含量及其抗氧化活性。分别采用水、40%乙醇及纤维素酶辅助乙醇超声提取蓝莓果渣，并用Folin-Ciocalteu试剂对3种提取物的总酚含量进行评估；并采用DPPH清除实验及O^—·₂清除实验对3种提取物的抗氧化活性进行研究。实验结果表明，纤维素酶辅助超声提取蓝莓果渣的总酚含量最高，可达425.36±15.21 mg GAE·100 g^-1 DW，远远高于水提物（169.46±9.75 mg GAE·100 g^-1 DW）及醇提物（218.39±12.54 mg GAE·100 g^-1 DW）中的总酚含量。且纤维素酶辅助乙醇超声提取物对DPPH的清除能力为2.67±0.13 g Vc·100 g^-1 DW，对O^—·₂的清除能力2.48±0.14 g Vc·100 g^-1 DW，明显好于醇提物及水提物抗氧化活性。

Abstract:Total phenolics and antioxidant activities of blueberry pomace extracts were studied in the present study. Water, 40% ethanol and cellulase-ultrasonic-assisted extraction were used to extract blueberry pomace in this study. Folin-Ciocalteu method was employed for total phenol contents determination. Moreover, the antioxidant activities of aqueous extracts, ethanol extracts and cellulase-ultrasonic-assisted extracts were determined by DPPH free radical scavenging assay and O^-·₂ free radical scavenging assay. Results showed that total phenolic content of cellulase-ultrasonic-assisted extracts (425.36±15.2 mg GAE·100 g^-1 DW) was much higher than that of aqueous extracts (169.46±9.7 mg GAE·100 g^-1 DW) and ethanol extracts (218.39±12.5 mg GAE·100 g^-1 DW). The antioxidant activity of cellulase-ultrasonic-assisted extracts in the DPPH radical scavenging and superoxide radical scavenging assay were 2.67±0.13 g Vc·100 g^-1 DW and 2.48±0.14 g Vc·100 g^-1 DW, respectively. These values were much lower than those of the aqueous extracts and ethanol extracts.

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