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作 者 ：孙红梅;赵爽;王春夏;陈丽静;王锦霞

期 刊 ：植物研究 2008年 28卷 5期 页码：594-598

关键词：兰州百合；鳞茎；苯丙氨酸解氨酶；提取技术；

Keywords:Lilium davidii var. unicolor, Bulb, Phenylalanine ammonia-lyase(PAL), extractive technique,

摘 要 ：研究兰州百合(Lilium davidii var. unicolor)鳞茎内PAL的最佳提取条件结果表明：pH 8.8的硼砂缓冲液为最适缓冲液；百合鳞茎的PAL不耐酸碱，更不耐酸；最适反应温度为40℃；随着水浴时间延长和水浴温度提高，PAL活性逐渐降低，但40℃水浴30 min后，酶活性仍保留47%；最适底物浓度为0.032 mol·L^-1；磨样时加入PVP 0.5 g，能够明显提高PAL活性。

Abstract:Optimum extractive technique of phenylalanine ammonia-lyase(PAL) in the bulb of Lilium davidii var. unicolor was studied. The results indicated that Borax solution with pH8.8 was the best buffer. PAL in lily bulb was not able to endure the strong acid and alkali, especially the former. The PAL activity declined gradually along with the increase of temperature and prolonging of bath duration, but the activity still remained 47% after 30 min in 40℃, and it was the optimum bath temperature. 0.032 mol·L^-1 substrate was the best, and grinding with 0.5 g PVP could improve the PAL activity obviously.

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