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作 者 ：金彦;姚晓慧;段明慧;张东阳;付玉杰;*

期 刊 ：植物研究 2013年 33卷 (3)期 页码：367-370

关键词：鹿蹄草；金丝桃苷；双水相辅助提取；Box-Behnken Design设计；

Keywords:Pyrola calliantha H.Andr., hyperin, aqueous two-phase system(ATPS) extraction, Box-Behnken design,

摘 要 ：以鹿蹄草为原料，利用微波双水相辅助提取技术进行提取，在单因素实验的基础上对提取条件进行了考察，根据BBD(BoxBehnken Design)实验设计原理，采用3因素3水平的响应面分析法，以鹿蹄草中金丝桃苷(hyperin)为指标，对提取过程进行优化，得到最佳工艺参数为:提取温度50℃,液固比36∶1，(NH₄)₂SO₄浓度22%，提取时间22 min，微波功率300 W，提取次数3次。在最佳提取条件下，金丝桃苷的提取率可达0.982 mg·g^-1，纯度可以达0.443%。

Abstract:Based on the preliminary experiments, a three-variable, three-level Box-Behnken design was performed to study the aqueous two-phase system (ATPS) extraction of hyperin from Pyrola calliantha H. Andr.. The optimum parameters were as follows: extraction temperature 50℃, liquid /solid ratio 36∶1, (NH₄)₂SO₄ concentration 22%, extraction time 22 min, extraction power 300 W and three extraction cycles. Under the optimal conditions, the average extraction yield of hyperin was 0.982 mg·g^-1 and the purity was 0.443%. This study is significant for the application of an aqueous two-phase system (ATPS) extraction technology and utilization of P.calliantha H. Andr..

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