Abstract:In this paper, the effects of temperature, lights, GA3 and NAA on the seeds germination of Swertia bimaculata were studied. The main results show that the favorable temperature for their germination is 15~20℃, the rate of seeds germination are enhanced by pretreated of low temperature, but the effects of lights is insignificant, both GA3 and NAA increase the vigor of germination, moreover, GA3 can improve the rates of germination. This study can be concluded that the seeds of S. bimaculata have dormancy, both hormone and 4℃ cold storage have important effective for dormancy breaking.