Abstract:The seeds of Sinopodophyllum hexandrum Royle were taken as materials. The effects of exogenous hormones on induction, multiplication and rooting of explants were discussed. The regeneration system of S.hexandrum has been established. The results showed that: The optimal conditions for shoot induction were 3.0 mg·L-1 6-BA+0.5 mg·L-1 GA-3. The induction rate was 85.71% with a shortening of 30-40 d. For multiplication, the optimal conditions were 3.0 mg·L-1 6-BA+0.2 mg·L-1 IAA, and the mean shoots obtained per explant was 1.63. The most proper rooting medium was: WPM+1.5 mg·L-1 IAA+0.5 mg·L-1 NAA and the rooting ratio was 60.1% after 30 d culturing.