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作 者 ：杨虎彪;李晓霞;罗丽娟*

期 刊 ：植物研究 2009年 29卷 2期 页码：242-244

关键词：禾本科；鲜叶；下表皮；

Keywords:Gramineae, fresh leaf, lower epidermis, method,

摘 要 ：介绍一种禾本科植物鲜叶下表皮制片的快速方法，该方法也能适用于多数单子叶植物鲜叶和经FAA固定液固定的单子叶植物叶片的气孔观察。该方法制片能有效防止叶表皮脱水、透明后发生卷曲，使保持完整的结构，便于准确的显微观察。具操作简单、材料易得、可靠性和稳定性高等优点。

Abstract:The aim of this study was to introduce an efficiect and fast method for observation the lower epidermis of fresh leaf of Gramineae, it also can be used for observation the stomata of Monocotyledon whatever fresh leaf or have been fixed in FAA solution. This method can avoid crispation when epidermis dehydration in 100% ethanol and clearing in Xylene to keep the full framework for accurate and efficient observation of microstructure. Furthermore, this method exhibits higher reliability and stability for micromorphology research of leaf epidermis of Gramineae and Monocotyledon.

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